This week, VMRD announced the availability of a substrate slide that can be used for the detection of antibody to Coxiella burnetii (Q fever). The slides are of twelve-well, Teflon-masked format.
Each well contains two separate Teflon rings delineating phase I and phase II of the bacterium.
Within each ring, phase - specific C. burnetii organisms are embedded in a pre-counterstained yolk substrate yielding sparkling green fluorescence with positive samples and a faint orange or red background with negati ve samples that helps to confirm that the observation is taking place in the correct focal plane.
“With our new Q Fever IFA offering we’ve made a number of usability enhancements over alternative IFA products on the market while maintaining a reasonable price point,” said Ethan Adams, Vice President of VMRD. “I believe our slides are the best value among the several options presently available to the veterinary diagnostic lab community.”
VMRD ’s FA substrate slide is designed for use as an antibody dete ction tool and can be used both qualitatively (positive or negative result) and semi-quantitatively (antibody titration).
This tool has potential value for screening and surveill ance and can also be used to evaluate the antibody levels of infected populations of cows, goats, and sheep. Positive and negative controls are also available.
Coxiella burnetii is an intracellular bacterium that is the causative agent for Q fever and was first described in 1937. It has been found worldwide with cattle, sheep and goats as the primary reservoirs, though it may infect many other species. The organism can survive for long periods of time in the environment as it is resistant to heat, drying, and many common disinfe ctants.
Serologic testing can evaluate presence of antibodies to both phase I (chronic stage) and phase II (acute stage) of C. burnetii. Other testing methods include PCR, culture isolation, and immunohistochemistry.
For more information, contact VMRD at 509 - 334 - 5815 or visit www.vmrd.com.